Electrospun Fe3O4/TiO2 hybrid nanofibers and their in vitro biocompatibility: prospective matrix for satellite cell adhesion and cultivation.

ABSTRACT

We report the fabrication of novel Fe3O4/TiO2 hybrid nanofibers with the improved cellular response for potential tissue engineering applications. In this study, Fe3O4/TiO2 hybrid nanofibers were prepared by facile sol-gel electrospinning using titanium isopropoxide and iron(III) nitrate nonahydrate as precursors. The obtained electrospun nanofibers were vacuum dried at 80 °C and then calcined at 500 °C. The physicochemical characterization of the synthesized composite nanofibers was carried out by scanning electron microscopy, energy dispersive X-ray spectroscopy, transmission electron microscopy and X-ray diffraction pattern. To examine the in vitro cytotoxicity, satellite cells were treated with as-prepared Fe3O4/TiO2 and the viability of cells was analyzed by Cell Counting Kit-8 assay at regular time intervals. The morphological features of unexposed satellite cells and exposed to Fe3O4/TiO2 composite were examined with a phase contrast microscope whereas the quantification of cell viability was carried out via confocal laser scanning microscopy. The morphology of the cells attached to hybrid matrix was observed by Bio-SEM. Cytotoxicity experiments indicated that the satellite cells could attach to the Fe3O4/TiO2 composite nanofibers after being cultured. We observed that Fe3O4-TiO2 composite nanofibers could support cell adhesion and growth. Results from this study therefore suggest that Fe3O4/TiO2 composite scaffold with small diameters (approximately 200 nm) can mimic the natural extracellular matrix well and provide possibilities for diverse applications in the field of tissue engineering and regenerative medicine.