Ontogeny of glutamate accumulating activity in rat brain synaptic vesicles.
Neurosci Lett
; 97(1-2): 185-90, 1989 Feb 13.
Article
in En
| MEDLINE
| ID: mdl-2563905
ABSTRACT
The relationship between the ontogeny of the vesicular glutamate uptake system and synaptogenesis in rats was investigated. For this purpose we have developed a simplified procedure for the preparation of crude synaptic vesicles which are sufficiently pure to demonstrate a highly ATP-dependent glutamate uptake. ATP-dependent glutamate uptake into synaptic vesicles was found to increase dramatically starting on postnatal day 10 and reaching a maximum on day 30 (76 +/- 40 and 657 +/- 40 pmol/mg protein/10 min, respectively), correlating well with the active period of synaptogenesis. Stimulation of uptake by chloride also developed in parallel with the vesicular glutamate uptake. In contrast, combined non-ATP-dependent uptake and non-specific binding remained constant (21 +/- 6 pmol/mg protein/10 min). This development of vesicular glutamate uptake during the period of synaptogenesis supports the notion that synaptic vesicles play an important role in glutamate synaptic transmission.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Synaptic Vesicles
/
Brain
/
Aging
/
Glutamates
/
Membrane Proteins
Limits:
Animals
Language:
En
Journal:
Neurosci Lett
Year:
1989
Document type:
Article