The ABBA motif binds APC/C activators and is shared by APC/C substrates and regulators.
Dev Cell
; 32(3): 358-372, 2015 Feb 09.
Article
in En
| MEDLINE
| ID: mdl-25669885
ABSTRACT
The anaphase-promoting complex or cyclosome (APC/C) is the ubiquitin ligase that regulates mitosis by targeting specific proteins for degradation at specific times under the control of the spindle assembly checkpoint (SAC). How the APC/C recognizes its different substrates is a key problem in the control of cell division. Here, we have identified the ABBA motif in cyclin A, BUBR1, BUB1, and Acm1, and we show that it binds to the APC/C coactivator CDC20. The ABBA motif in cyclin A is required for its proper degradation in prometaphase through competing with BUBR1 for the same site on CDC20. Moreover, the ABBA motifs in BUBR1 and BUB1 are necessary for the SAC to work at full strength and to recruit CDC20 to kinetochores. Thus, we have identified a conserved motif integral to the proper control of mitosis that connects APC/C substrate recognition with the SAC.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protein Serine-Threonine Kinases
/
Ubiquitin-Protein Ligase Complexes
/
Anaphase-Promoting Complex-Cyclosome
/
Cdc20 Proteins
/
Membrane Proteins
/
Microfilament Proteins
/
Mitosis
Limits:
Humans
Language:
En
Journal:
Dev Cell
Journal subject:
EMBRIOLOGIA
Year:
2015
Document type:
Article
Affiliation country:
Reino Unido