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G&T-seq: parallel sequencing of single-cell genomes and transcriptomes.
Macaulay, Iain C; Haerty, Wilfried; Kumar, Parveen; Li, Yang I; Hu, Tim Xiaoming; Teng, Mabel J; Goolam, Mubeen; Saurat, Nathalie; Coupland, Paul; Shirley, Lesley M; Smith, Miriam; Van der Aa, Niels; Banerjee, Ruby; Ellis, Peter D; Quail, Michael A; Swerdlow, Harold P; Zernicka-Goetz, Magdalena; Livesey, Frederick J; Ponting, Chris P; Voet, Thierry.
Affiliation
  • Macaulay IC; Sanger Institute-EBI Single-Cell Genomics Centre, Wellcome Trust Sanger Institute, Hinxton, UK.
  • Haerty W; MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
  • Kumar P; Department of Human Genetics, University of Leuven, Leuven, Belgium.
  • Li YI; MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
  • Hu TX; MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
  • Teng MJ; Cancer Genome Project, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
  • Goolam M; Department of Physiology, Development and Neuroscience, Downing Site, University of Cambridge, Cambridge, UK.
  • Saurat N; Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK.
  • Coupland P; Sequencing R&D, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
  • Shirley LM; Sequencing R&D, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
  • Smith M; Sequencing R&D, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
  • Van der Aa N; Department of Human Genetics, University of Leuven, Leuven, Belgium.
  • Banerjee R; Cytogenetics Core Facility, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
  • Ellis PD; Sequencing R&D, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
  • Quail MA; Sequencing R&D, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
  • Swerdlow HP; Sequencing R&D, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.
  • Zernicka-Goetz M; Department of Physiology, Development and Neuroscience, Downing Site, University of Cambridge, Cambridge, UK.
  • Livesey FJ; Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK.
  • Ponting CP; 1] Sanger Institute-EBI Single-Cell Genomics Centre, Wellcome Trust Sanger Institute, Hinxton, UK. [2] MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
  • Voet T; 1] Sanger Institute-EBI Single-Cell Genomics Centre, Wellcome Trust Sanger Institute, Hinxton, UK. [2] Department of Human Genetics, University of Leuven, Leuven, Belgium.
Nat Methods ; 12(6): 519-22, 2015 Jun.
Article in En | MEDLINE | ID: mdl-25915121
ABSTRACT
The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA / RNA, Messenger / Nucleic Acid Amplification Techniques / Genomics Limits: Animals / Humans Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2015 Document type: Article Affiliation country: Reino Unido
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA / RNA, Messenger / Nucleic Acid Amplification Techniques / Genomics Limits: Animals / Humans Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2015 Document type: Article Affiliation country: Reino Unido