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A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.
Torres, Nikko P; Brown, Grant W.
Affiliation
  • Torres NP; Department of Biochemistry and Donnelly Centre, University of Toronto, 160 College Street, Toronto, ON, Canada, M5S 3E1.
Methods Mol Biol ; 1300: 1-12, 2015.
Article in En | MEDLINE | ID: mdl-25916702
ABSTRACT
High-throughput imaging of yeast cells expressing fluorescent proteins can be used to understand biological pathways in the context of spatial organization. Here we describe a method for imaging yeast cells expressing proteins tagged with green fluorescent protein (GFP) and/or red fluorescent protein (RFP), with or without drug treatment, in a 384-well format, using the PerkinElmer Opera high-content confocal imaging microscope.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Saccharomyces cerevisiae / Stress, Physiological / Microscopy, Confocal / DNA Replication / Microscopy, Fluorescence Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2015 Document type: Article
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Saccharomyces cerevisiae / Stress, Physiological / Microscopy, Confocal / DNA Replication / Microscopy, Fluorescence Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2015 Document type: Article