Rapid evolution of in vivo-selected sequences and structures replacing 20% of a subviral RNA.
Virology
; 483: 149-62, 2015 Sep.
Article
in En
| MEDLINE
| ID: mdl-25974866
ABSTRACT
The 356 nt noncoding satellite RNA C (satC) of Turnip crinkle virus (TCV) is composed of 5' sequences from a second TCV satRNA (satD) and 3' sequences derived from TCV. SHAPE structure mapping revealed that 76 nt in the poorly-characterized satD-derived region form an extended hairpin (H2). Pools of satC in which H2 was replaced with 76, 38, or 19 random nt were co-inoculated with TCV helper virus onto plants and satC fitness assessed using in vivo functional selection (SELEX). The most functional progeny satCs, including one as fit as wild-type, contained a 38-39 nt H2 region that adopted a hairpin structure and exhibited an increased ratio of dimeric to monomeric molecules. Some progeny of satC with H2 deleted featured a duplication of 38 nt, partially rebuilding the deletion. Therefore, H2 can be replaced by a 38-39 nt hairpin, sufficient for overall structural stability of the 5' end of satC.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Selection, Genetic
/
Evolution, Molecular
/
Carmovirus
/
RNA, Satellite
Language:
En
Journal:
Virology
Year:
2015
Document type:
Article
Affiliation country:
Estados Unidos