Preparation of vesicular stomatitis virus pseudotype with Chikungunya virus envelope protein.
Acta Virol
; 59(2): 189-93, 2015 Jun.
Article
in En
| MEDLINE
| ID: mdl-26104337
Key words
Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus that causes Chikungunya fever (CHIKF) in millions of people mainly in developing countries. CHIKF is characterized by high fever; efficient and easy neutralization assay of CHIKV based on vesicular stomatitis virus (VSV) pseudotype with CHIKV envelope protein and the green fluorescent protein (GFP) or luciferase as reporter gene; fatigue; headache; myalgia and severe arthralgia. To date; nausea; rash; there is no specific treatment and no licensed vaccine against CHIKV infection. In this study; vomiting; we developed a safe; which could be used under a reduced safety level. The VSV pseudotype can be applied to the epidemic survey by measuring the expression of GFP or luciferase activity in infected cells. This system can also be used to study the mechanisms of virus entry.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chikungunya virus
/
Viral Envelope Proteins
/
Vesicular stomatitis Indiana virus
/
Chikungunya Fever
Limits:
Animals
/
Humans
Language:
En
Journal:
Acta Virol
Year:
2015
Document type:
Article
Country of publication:
Eslovaquia