An Escherichia coli system for evolving improved light-controlled DNA-binding proteins.
Protein Eng Des Sel
; 28(9): 293-302, 2015 Sep.
Article
in En
| MEDLINE
| ID: mdl-26245690
ABSTRACT
Light-switchable proteins offer numerous opportunities as tools for manipulating biological systems with exceptional degrees of spatiotemporal control. Most designed light-switchable proteins currently in use have not been optimised using the randomisation and selection/screening approaches that are widely used in other areas of protein engineering. Here we report an approach for screening light-switchable DNA-binding proteins that relies on light-dependent repression of the transcription of a fluorescent reporter. We demonstrate that the method can be used to recover a known light-switchable DNA-binding protein from a random library.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transcription, Genetic
/
Protein Engineering
/
DNA-Binding Proteins
Type of study:
Clinical_trials
Language:
En
Journal:
Protein Eng Des Sel
Journal subject:
BIOQUIMICA
/
BIOTECNOLOGIA
Year:
2015
Document type:
Article