Quantification of insulin receptor mRNA splice variants as a diagnostic tumor marker in breast cancer.
Cancer Biomark
; 15(5): 653-61, 2015.
Article
in En
| MEDLINE
| ID: mdl-26406954
ABSTRACT
BACKGROUND:
The mature human insulin receptor (INSR) has two isoforms The A isoform and the B isoform. INSR upregulation has been suggested to play a role in cancer.OBJECTIVE:
To establish quantitative PCR method for INSR transcript variants and examine their differential expression as a diagnostic tumor marker in breast cancer.METHODS:
The differential expression of IR-A and IR-B were evaluated by TaqMan qRT-PCR assay in the commercially available Breast Cancer Disease cDNA and Cancer Survey cDNA arrays.RESULTS:
The mRNA expression levels of IR-A was statistically significantly higher in breast cancer when compared to normal breast tissue while IR-B mRNA expression was down regulated significantly in breast cancer. Stratification of patients into groups according to metastatic stages indicated statistically significantly higher levels of IR-A mRNA in clinical stage (CS)-IV, and lower IR-B levels in CS-IIA, CS-IIIB and CS-IIIC. However, IR-AIR-B ratio showed a statistically significant increase in all stages. Cancer Survey cDNA array demonstrated lower levels of IR-B mRNA in breast adenocarcinoma, liver carcinoma and lung carcinoma only while IR-A expression was significantly altered in kidney carcinoma without any significant differences in IR-AIR-B ratios.CONCLUSIONS:
The results demonstrate an increased IR-AIR-B ratio in all clinical stages of breast cancer. Thus, IR-AIR-B ratio may have a diagnostic biomarker utility in breast cancer.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Breast Neoplasms
/
Receptor, Insulin
/
Biomarkers, Tumor
/
Antigens, CD
/
Protein Isoforms
Type of study:
Diagnostic_studies
Limits:
Female
/
Humans
Language:
En
Journal:
Cancer Biomark
Journal subject:
BIOQUIMICA
/
NEOPLASIAS
Year:
2015
Document type:
Article