Generation of a recombinant West Nile virus stably expressing the Gaussia luciferase for neutralization assay.
Virus Res
; 211: 17-24, 2016 Jan 04.
Article
in En
| MEDLINE
| ID: mdl-26415755
ABSTRACT
West Nile virus (WNV) is a neurotropic human pathogen that has caused increasing infected cases over recent years. There is currently no licensed vaccine or effective drug for prevention and treatment of WNV infection in humans. To facilitate antiviral drug discovery and neutralizing antibody detection, a WNV cDNA clone containing a luciferase reporter gene was constructed through incorporating Gaussia luciferase (Gluc) gene within the capsid-coding region of WNV genome. Transfection of BHK-21 cells with the cDNA clone-derived RNA generated luciferase reporter WNV (WNV-Gluc) and the stable WNV-Gluc with high titers (>10(7)PFU/ml) was obtained through plaque purification. Luciferase activity was used to effectively quantify the viral production of WNV-Gluc. Using the reporter virus WNV-Gluc, we developed a luciferase based assay in a 12-well format for evaluating neutralizing antibodies. The reporter virus could be a powerful tool for epidemiological investigation of WNV, vaccine evaluation, antiviral drug screening, and the study of WNV replication and pathogenesis.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
West Nile virus
/
Neutralization Tests
/
Antibodies, Neutralizing
/
Luciferases
Type of study:
Evaluation_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Virus Res
Journal subject:
VIROLOGIA
Year:
2016
Document type:
Article
Affiliation country:
China