Evaluation of antifertility effect of gel formulation containing sperm immobilizing factor: In vitro and in vivo studies.
Eur J Pharm Sci
; 81: 67-74, 2016 Jan 01.
Article
in En
| MEDLINE
| ID: mdl-26455286
ABSTRACT
Sexually active women often seek protection against unplanned pregnancies. Latter can be effectively controlled by consistent use of spermicides during each coital act. However, side effects associated with the use of available synthetic spermicidal agents have directed the interest towards identifying newer and safer agents. Present studies were undertaken to formulate a vaginal contraceptive gel, containing sperm immobilizing factor (SIF) isolated from Staphylococcus aureus using 1% w/v Carbopol. SIF loaded gel formulation was characterized for various in vitro parameters i.e. pH, spreadability, texture profiling, rheological properties, and in vitro release studies. The prepared formulation was found to possess significant spreading properties, gel firmness and strength, and released about 80% of SIF within 30min. Latter can completely immobilize human spermatozoa within 20s, at a dose of 200µg/ml. SIF in the proposed gel formulation showed 100% contraceptive effect when used at amount as low as of 10µg, thus confirming the possibility to develop it as a potent vaginal contraceptive for future use.
Key words
Ammonium sulfate (PubChem CID: 6097028); Carbopol; Carbopol (PubChem CID: 6581); Contraception; Controlled release; Diethylaminoethyl-cellulose (PubChem CID: 16211032); Disodium hydrogen phosphate (PubChem CID: 24203); Sodium chloride (PubChem CID: 5234); Sodium dihydrogen orthophosphate (PubChem CID: 23672064); Sperm immobilization; Staphylococcus aureus
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sperm Immobilizing Agents
/
Spermatozoa
/
Fertility
Type of study:
Prognostic_studies
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
En
Journal:
Eur J Pharm Sci
Journal subject:
FARMACIA
/
FARMACOLOGIA
/
QUIMICA
Year:
2016
Document type:
Article
Affiliation country:
India
Publication country:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS