Epigallocatechin-3-gallate attenuates the AIM2-induced secretion of IL-1ß in human epidermal keratinocytes.
Biochem Biophys Res Commun
; 467(4): 723-9, 2015 Nov 27.
Article
in En
| MEDLINE
| ID: mdl-26494301
ABSTRACT
The pro-inflammatory cytokine interleukin-1ß (IL-1ß) plays a central role in the pathogenesis of psoriasis. Keratinocytes are a major source of IL-1ß and express absent in melanoma 2 (AIM2). AIM2 recognizes a double-stranded DNA and initiates the IL-1ß-processing of inflammasome. The AIM2 inflammasome is a cytosolic multiprotein complex composed of AIM2, an apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), and pro-caspase-1. Epigallocatechin-3-Gallate (EGCG), a major polyphenolic component of green tea, has anti-inflammatory properties. In the current study, we investigated the issue of whether or how EGCG suppresses AIM2 inflammasome in human epidermal keratinocytes, neonatal (HEKn). Treatment with EGCG, before or after IFN-γ priming, attenuated poly(dAdT)-induced IL-1ß secretion in HEKn cells. Pre-treatment with EGCG reduced the level of IFN-γ-induced priming signal via the down-regulation of pro-IL-1ß and pro-capspase-1 in HEKn cells. Furthermore, treatment with EGCG attenuated poly(dAdT)-induced ASC oligomerization and caspase-1 activation in IFN-γ-primed HEKn cells. These results suggest that EGCG attenuates AIM2-induced IL-1ß secretion by suppressing both IFN-γ-mediated priming and poly(dAdT)-induced ASC oligomerization of inflammasomes in human epidermal keratinocytes.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Catechin
/
Keratinocytes
/
DNA-Binding Proteins
/
Interleukin-1beta
Limits:
Humans
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2015
Document type:
Article
Affiliation country:
Corea del Sur