[Effect of Intestinal Ischemia-reperfusion Injury on the Expression of Chemerin in Mice].

ABSTRACT

OBJECTIVE: To investigate the effect of intestinal ischemia-reperfusion on the protein expression of chemerin in C57BL/6J mice. METHODS: A total of 16 mice (C57BL/6J, specific pathogen free level) were randomly assigned into two groups (n=8 each) the intestinal ischemia-reperfusion group and the sham group. The intestinal ischemia-reperfusion state was achieved by blocking the super-mesenteric artery. After 60 minutes of ischemia followed by 60 minutes of reperfusion,we determined the protein level of chemerin in various organs and tissues by enzyme-linked immunosorbent assay and Western blot. The sham group underwent the same operation process except for the blocking of the super-mesenteric artery. RESULT: The protein level of chemerin was significantly elevated in distinctive organs and tissues in the state of intestinal ischemia-reperfusion (P<0.05). CONCLUSION: The intestinal ischemia-reperfusion can remarkably increase the protein expression of chemerin in some organs and tissues.