Schistosoma mansoni α1,3-fucosyltransferase-F generates the Lewis X antigen.
Glycobiology
; 26(3): 270-85, 2016 Mar.
Article
in En
| MEDLINE
| ID: mdl-26582608
ABSTRACT
Genetic evidence suggests that the Schistosoma mansoni genome contains six genes that encode α1,3-fucosyltransferases (smFuTs). To date, the activities and specificities of these putative fucosyltransferases are unknown. As Schistosoma express a variety of fucosylated glycans, including the Lewis X antigen Galß1-4(Fucα1-3)GlcNAcß-R, it is likely that this family of genes encode enzymes that are partly responsible for the generation of those structures. Here, we report the molecular cloning of fucosyltransferase-F (smFuT-F) from S. mansoni, as a soluble, green fluorescent protein fusion protein and its acceptor specificity. The gene smFuT-F was expressed in HEK freestyle cells, purified by affinity chromatography, and analyzed toward a broad panel of glycan acceptors. The enzyme product of smFuT-F effectively utilizes a type II chain acceptor Galß1-4GlcNAc-R, but notably not the LDN sequence GalNAcß1-4GlcNAc-R, to generate Lewis X type-glycans, and smFuT-F transcripts are present in all intramammalian life stages.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polysaccharides
/
Schistosoma mansoni
/
Lewis X Antigen
/
Fucosyltransferases
Limits:
Animals
Language:
En
Journal:
Glycobiology
Journal subject:
BIOQUIMICA
Year:
2016
Document type:
Article
Affiliation country:
Estados Unidos