Comparison of the Efficiency of Viral Transduction and Episomal Transfection in Human Fibroblast Reprogramming.

Vdovin, A S; Lupatov, A Yu; Kholodenko, I V; Yarygin, K N

Vdovin, A S; Lupatov, A Yu; Kholodenko, I V; Yarygin, K N.

Affiliation

Vdovin AS; V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
Lupatov AY; V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
Kholodenko IV; V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia. irkhol@yandex.ru.
Yarygin KN; V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.

Bull Exp Biol Med ; 160(1): 123-8, 2015 Nov.

Article in En | MEDLINE | ID: mdl-26593412

ABSTRACT

ABSTRACT

Induced pluripotent cells were derived from adult human skin fibroblast by using two methods of reprogramming. Episomal transfection with vectors containing oriP/EBNA-1 sequence for delivery of reprogramming genes Oct4, Sox2, Klf4, L-Myc, and Lin28 proved to be more effective than viral transduction with Sendai virus-based vector ~200 and 8 colonies per 10(5) cells were found on day 21 of culturing, respectively. Colonies of induced pluripotent cells obtained by these two methods expressed pluripotency marker Tra1-60.

Subject(s)
Key words

differentiation; induced pluripotent stem cells; reprogramming; transduction; transfection

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plasmids / Transduction, Genetic / Transfection / Induced Pluripotent Stem Cells / Fibroblasts / Cellular Reprogramming Techniques Limits: Adult / Humans / Male Language: En Journal: Bull Exp Biol Med Year: 2015 Document type: Article Affiliation country: Rusia

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