Note: Time-gated 3D single quantum dot tracking with simultaneous spinning disk imaging.
Rev Sci Instrum
; 86(12): 126102, 2015 Dec.
Article
in En
| MEDLINE
| ID: mdl-26724083
ABSTRACT
We describe recent upgrades to a 3D tracking microscope to include simultaneous Nipkow spinning disk imaging and time-gated single-particle tracking (SPT). Simultaneous 3D molecular tracking and spinning disk imaging enable the visualization of cellular structures and proteins around a given fluorescently labeled target molecule. The addition of photon time-gating to the SPT hardware improves signal to noise by discriminating against Raman scattering and short-lived fluorescence. In contrast to camera-based SPT, single-photon arrival times are recorded, enabling time-resolved spectroscopy (e.g., measurement of fluorescence lifetimes and photon correlations) to be performed during single molecule/particle tracking experiments.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Image Enhancement
/
Imaging, Three-Dimensional
/
Quantum Dots
/
Molecular Imaging
/
Cell Tracking
/
Microscopy, Fluorescence
Type of study:
Diagnostic_studies
/
Evaluation_studies
Language:
En
Journal:
Rev Sci Instrum
Year:
2015
Document type:
Article
Affiliation country:
Estados Unidos