Your browser doesn't support javascript.
loading
Note: Time-gated 3D single quantum dot tracking with simultaneous spinning disk imaging.
DeVore, M S; Stich, D G; Keller, A M; Cleyrat, C; Phipps, M E; Hollingsworth, J A; Lidke, D S; Wilson, B S; Goodwin, P M; Werner, J H.
Affiliation
  • DeVore MS; Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Mail Stop G755, Los Alamos, New Mexico 87545, USA.
  • Stich DG; Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Mail Stop G755, Los Alamos, New Mexico 87545, USA.
  • Keller AM; Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Mail Stop G755, Los Alamos, New Mexico 87545, USA.
  • Cleyrat C; Department of Pathology and Cancer Research and Treatment Center, University of New Mexico, Albuquerque, New Mexico 87131, USA.
  • Phipps ME; Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Mail Stop G755, Los Alamos, New Mexico 87545, USA.
  • Hollingsworth JA; Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Mail Stop G755, Los Alamos, New Mexico 87545, USA.
  • Lidke DS; Department of Pathology and Cancer Research and Treatment Center, University of New Mexico, Albuquerque, New Mexico 87131, USA.
  • Wilson BS; Department of Pathology and Cancer Research and Treatment Center, University of New Mexico, Albuquerque, New Mexico 87131, USA.
  • Goodwin PM; Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Mail Stop G755, Los Alamos, New Mexico 87545, USA.
  • Werner JH; Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Mail Stop G755, Los Alamos, New Mexico 87545, USA.
Rev Sci Instrum ; 86(12): 126102, 2015 Dec.
Article in En | MEDLINE | ID: mdl-26724083
ABSTRACT
We describe recent upgrades to a 3D tracking microscope to include simultaneous Nipkow spinning disk imaging and time-gated single-particle tracking (SPT). Simultaneous 3D molecular tracking and spinning disk imaging enable the visualization of cellular structures and proteins around a given fluorescently labeled target molecule. The addition of photon time-gating to the SPT hardware improves signal to noise by discriminating against Raman scattering and short-lived fluorescence. In contrast to camera-based SPT, single-photon arrival times are recorded, enabling time-resolved spectroscopy (e.g., measurement of fluorescence lifetimes and photon correlations) to be performed during single molecule/particle tracking experiments.
Subject(s)
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Image Enhancement / Imaging, Three-Dimensional / Quantum Dots / Molecular Imaging / Cell Tracking / Microscopy, Fluorescence Type of study: Diagnostic_studies / Evaluation_studies Language: En Journal: Rev Sci Instrum Year: 2015 Document type: Article Affiliation country: Estados Unidos
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Image Enhancement / Imaging, Three-Dimensional / Quantum Dots / Molecular Imaging / Cell Tracking / Microscopy, Fluorescence Type of study: Diagnostic_studies / Evaluation_studies Language: En Journal: Rev Sci Instrum Year: 2015 Document type: Article Affiliation country: Estados Unidos