High performance dual-mode surface plasmon coupled emission imaging apparatus integrating Kretschmann and reverse Kretschmann configurations for flexible measurements.
Rev Sci Instrum
; 87(1): 013705, 2016 Jan.
Article
in En
| MEDLINE
| ID: mdl-26827326
ABSTRACT
A Kretschmann (KR) and reverse Kretschmann (RK) dual-mode surface plasmon coupled emission (SPCE) imaging apparatus based on prism coupling was built up. Highly directional and polarized fluorescence images for both RK and KR configurations were obtained. Besides, surface plasmon field-enhanced fluorescence and free space imaging can also be measured conveniently from this apparatus. Combining the high sensitivity of KR mode and the simplicity of RK mode, the multifunctional imaging system is flexible to provide different configurations for imaging applications. Compared to the free space imaging, SPCE imaging provides enhanced fluorescence, especially large enhancement up to about 50 fold in KR configuration. Additionally, the degree of evanescent field enhancement effect was easily estimated experimentally using the apparatus to compare the different imaging configurations. We believed that the dual-mode SPCE imaging apparatus will be useful in fundamental study of plasmon-controlled fluorescence and be a powerful tool for optical imaging, especially for microarray and biological applications.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Surface Plasmon Resonance
/
Molecular Imaging
Language:
En
Journal:
Rev Sci Instrum
Year:
2016
Document type:
Article
Affiliation country:
China