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High performance dual-mode surface plasmon coupled emission imaging apparatus integrating Kretschmann and reverse Kretschmann configurations for flexible measurements.
Cai, Wei-Peng; Zhai, Yan-Yun; Cao, Shuo-Hui; Liu, Qian; Weng, Yu-Hua; Xie, Kai-Xin; Lin, Guo-Chun; Li, Yao-Qun.
Affiliation
  • Cai WP; Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
  • Zhai YY; Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
  • Cao SH; Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
  • Liu Q; Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
  • Weng YH; Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
  • Xie KX; Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
  • Lin GC; School of Physics and Mechanical & Electrical Engineering, Xiamen University, Xiamen 361005, China.
  • Li YQ; Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Rev Sci Instrum ; 87(1): 013705, 2016 Jan.
Article in En | MEDLINE | ID: mdl-26827326
ABSTRACT
A Kretschmann (KR) and reverse Kretschmann (RK) dual-mode surface plasmon coupled emission (SPCE) imaging apparatus based on prism coupling was built up. Highly directional and polarized fluorescence images for both RK and KR configurations were obtained. Besides, surface plasmon field-enhanced fluorescence and free space imaging can also be measured conveniently from this apparatus. Combining the high sensitivity of KR mode and the simplicity of RK mode, the multifunctional imaging system is flexible to provide different configurations for imaging applications. Compared to the free space imaging, SPCE imaging provides enhanced fluorescence, especially large enhancement up to about 50 fold in KR configuration. Additionally, the degree of evanescent field enhancement effect was easily estimated experimentally using the apparatus to compare the different imaging configurations. We believed that the dual-mode SPCE imaging apparatus will be useful in fundamental study of plasmon-controlled fluorescence and be a powerful tool for optical imaging, especially for microarray and biological applications.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Surface Plasmon Resonance / Molecular Imaging Language: En Journal: Rev Sci Instrum Year: 2016 Document type: Article Affiliation country: China
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Surface Plasmon Resonance / Molecular Imaging Language: En Journal: Rev Sci Instrum Year: 2016 Document type: Article Affiliation country: China