MB3W1 is an orthotopic xenograft model for anaplastic medulloblastoma displaying cancer stem cell- and Group 3-properties.
BMC Cancer
; 16: 115, 2016 Feb 17.
Article
in En
| MEDLINE
| ID: mdl-26883117
ABSTRACT
BACKGROUND:
Medulloblastoma is the most common malignant brain tumor in children and can be divided in different molecular subgroups. Patients whose tumor is classified as a Group 3 tumor have a dismal prognosis. However only very few tumor models are available for this subgroup.METHODS:
We established a robust orthotopic xenograft model with a cell line derived from the malignant pleural effusions of a child suffering from a Group 3 medulloblastoma.RESULTS:
Besides classical characteristics of this tumor subgroup, the cells display cancer stem cell characteristics including neurosphere formation, multilineage differentiation, CD133/CD15 expression, high ALDH-activity and high tumorigenicity in immunocompromised mice with xenografts exactly recapitulating the original tumor architecture.CONCLUSIONS:
This model using unmanipulated, human medulloblastoma cells will enable translational research, specifically focused on Group 3 medulloblastoma.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Medulloblastoma
/
Neoplasms, Experimental
Type of study:
Prognostic_studies
Limits:
Animals
/
Female
/
Humans
/
Infant
/
Male
Language:
En
Journal:
BMC Cancer
Journal subject:
NEOPLASIAS
Year:
2016
Document type:
Article
Affiliation country:
Alemania