Surfactant Protein-D Is Essential for Immunity to Helminth Infection.
PLoS Pathog
; 12(2): e1005461, 2016 Feb.
Article
in En
| MEDLINE
| ID: mdl-26900854
ABSTRACT
Pulmonary epithelial cell responses can enhance type 2 immunity and contribute to control of nematode infections. An important epithelial product is the collectin Surfactant Protein D (SP-D). We found that SP-D concentrations increased in the lung following Nippostrongylus brasiliensis infection; this increase was dependent on key components of the type 2 immune response. We carried out loss and gain of function studies of SP-D to establish if SP-D was required for optimal immunity to the parasite. N. brasiliensis infection of SP-D-/- mice resulted in profound impairment of host innate immunity and ability to resolve infection. Raising pulmonary SP-D levels prior to infection enhanced parasite expulsion and type 2 immune responses, including increased numbers of IL-13 producing type 2 innate lymphoid cells (ILC2), elevated expression of markers of alternative activation by alveolar macrophages (alvM) and increased production of the type 2 cytokines IL-4 and IL-13. Adoptive transfer of alvM from SP-D-treated parasite infected mice into naïve recipients enhanced immunity to N. brasiliensis. Protection was associated with selective binding by the SP-D carbohydrate recognition domain (CRD) to L4 parasites to enhance their killing by alvM. These findings are the first demonstration that the collectin SP-D is an essential component of host innate immunity to helminths.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Macrophages, Alveolar
/
Strongylida Infections
/
Pulmonary Surfactant-Associated Protein D
/
Epithelial Cells
/
Lung
/
Nippostrongylus
Limits:
Animals
Language:
En
Journal:
PLoS Pathog
Year:
2016
Document type:
Article
Affiliation country:
Sudáfrica