Development of an HPLC Method for the Determination of Ceftolozane/Tazobactam in Biological and Aqueous Matrixes.
J Chromatogr Sci
; 54(6): 1037-40, 2016 Jul.
Article
in En
| MEDLINE
| ID: mdl-27048639
ABSTRACT
Herein, we report the development and validation of an HPLC method to analyze ceftolozane and tazobactam simultaneously in human plasma, human serum, swine serum and saline matrixes. A reversed-phase column was used with a UV detector set at 260 nm and switched to 218 nm. The mobile phase consisted of methanol and sodium phosphate buffer at a flow rate of 1.1 mL/min. Cefepime was used as the internal standard. The standard curves were linear over a range of 0.4-50 µg/mL. This methodology represents a simple, reproducible approach to the determination of drug concentrations with sufficient accuracy and precision for pharmacokinetic studies undertaken with this recently FDA-approved antimicrobial therapy.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cephalosporins
/
Chemistry Techniques, Analytical
/
Chromatography, High Pressure Liquid
/
Penicillanic Acid
Limits:
Animals
/
Humans
Language:
En
Journal:
J Chromatogr Sci
Year:
2016
Document type:
Article
Affiliation country:
Estados Unidos
Publication country:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
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US
/
USA