In Vivo Biosynthesis of a ß-Amino Acid-Containing Protein.
J Am Chem Soc
; 138(16): 5194-7, 2016 04 27.
Article
in En
| MEDLINE
| ID: mdl-27086674
ABSTRACT
It has recently been reported that ribosomes from erythromycin-resistant Escherichia coli strains, when isolated in S30 extracts and incubated with chemically mis-acylated tRNA, can incorporate certain ß-amino acids into full length DHFR in vitro. Here we report that wild-type E. coli EF-Tu and phenylalanyl-tRNA synthetase collaborate with these mutant ribosomes and others to incorporate ß(3)-Phe analogs into full length DHFR in vivo. E. coli harboring the most active mutant ribosomes are robust, with a doubling time only 14% longer than wild-type. These results reveal the unexpected tolerance of E. coli and its translation machinery to the ß(3)-amino acid backbone and should embolden in vivo selections for orthogonal translational machinery components that incorporate diverse ß-amino acids into proteins and peptides. E. coli harboring mutant ribosomes may possess the capacity to incorporate many non-natural, non-α-amino acids into proteins and other sequence-programmed polymeric materials.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phenylalanine
/
Protein Engineering
/
Peptide Elongation Factor Tu
/
Escherichia coli Proteins
/
Amino Acyl-tRNA Synthetases
Language:
En
Journal:
J Am Chem Soc
Year:
2016
Document type:
Article
Affiliation country:
Estados Unidos