Sweeping of hydrophobic amines under inhomogeneous electric field and low surfactant concentration in micellar electrokinetic chromatography.
Electrophoresis
; 37(9): 1161-5, 2016 05.
Article
in En
| MEDLINE
| ID: mdl-27135308
ABSTRACT
The influence of sample matrix on sample sweeping in MEKC was examined in the presented manuscript. Significant focusing effect was observed for relatively hydrophobic cationic compounds (emetine, strychnine and quinine) using high ionic strength sample matrix (900 mM H3 PO4 /720 mM Tris) which conductivity was about ninefold higher than utilized BGE. Moreover, the results were obtained using BGE composed of comparatively low surfactant concentration (10 mM SDS) and 40 mM H3 PO4 /32 mM Tris buffer solution. About 200 to 300-fold preconcentration of analytes was reached with the presented method. Basing on experimental results and computer simulation using Simul5 software, hypothetical mechanism of observed phenomenon was proposed.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Surface-Active Agents
/
Chromatography, Micellar Electrokinetic Capillary
Language:
En
Journal:
Electrophoresis
Year:
2016
Document type:
Article
Affiliation country:
Polonia