Prevalence of human papillomavirus types and variants and p16(INK4a) expression in head and neck squamous cells carcinomas in São Paulo, Brazil.
Infect Agent Cancer
; 11: 20, 2016.
Article
in En
| MEDLINE
| ID: mdl-27148398
ABSTRACT
BACKGROUND:
Human papillomavirus (HPV) prevalence in head and neck squamous cell carcinomas (HNSCC) diverges geographically. The reliability of using p16(INK4a) expression as a marker of viral infection is controversial in HNSCC. We evaluated HPV types and HPV-16 variants prevalence, and p16(INK4a) expression in HNSCC specimens provided by two different Institutions in São Paulo.METHODS:
HPV DNA from formalin-fixed specimens was accessed by Inno-LiPA, HPV-16 variants by PCR-sequencing, and p16(INK4a) protein levels by immunohistochemistry.RESULTS:
Overall, HPV DNA was detected among 19.4 % of the specimens (36/186). Viral prevalence was higher in the oral cavity (25.0 %, 23/92) then in other anatomical sites (oropharynx 14,3 %, larynx 13.7 %) when samples from both Institutions were analyzed together. HPV prevalence was also higher in the oral cavity when samples from both Institutions were analyzed separately. HPV-16 was the most prevalent type identified in 69.5 % of the HPV positive smaples and specimens were assigned into Asian-American (57.2 %) or European (42.8 %) phylogenetic branches. High expression of p16(INK4a) was more common among HPV positive tumors.CONCLUSION:
Our results support a role for HPV-16 in a subset of HNSCC.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Type of study:
Prevalence_studies
/
Risk_factors_studies
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Infect Agent Cancer
Year:
2016
Document type:
Article
Affiliation country:
Brasil