Three Novel Alkaloids from Portulaca oleracea L. and Their Anti-inflammatory Effects.
J Agric Food Chem
; 64(29): 5837-44, 2016 Jul 27.
Article
in En
| MEDLINE
| ID: mdl-27396870
ABSTRACT
Three novel carbon skeleton alkaloids, named oleracimine (1), oleracimine A (2), and oleracone A (3), with one novel azulene carbon skeleton compound, oleracone B (4), and one known compound, ß-carboline (5), were first isolated from Portulaca oleracea L. The structures were determined using spectroscopic methods, including one- and two-dimensional nuclear magnetic resonance and high-resolution electrospray ionization time-of-flight mass spectrometry techniques. In addition, oleracimine (1) was used to investigate the anti-inflammatory effects on lipopolysaccharide-stimulated macrophages. The results of enzyme-linked immunosorbent assay, western blot, and real-time polymerase chain reaction showed that oleracimine (1) remarkably inhibited nitric oxide production and could dose-dependently decrease the secretions of interleukin 6, tumor necrosis factor α, nitric oxide, and prostaglandin E2 in cell culture supernatants as well as the mRNA of cyclooxygenase-2 and inducible nitric oxide synthase.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Extracts
/
Portulaca
/
Alkaloids
/
Anti-Inflammatory Agents
Limits:
Animals
Language:
En
Journal:
J Agric Food Chem
Year:
2016
Document type:
Article