Double-edged-sword effect of IL-1ß on the osteogenesis of periodontal ligament stem cells via crosstalk between the NF-κB, MAPK and BMP/Smad signaling pathways.
Cell Death Dis
; 7: e2296, 2016 07 14.
Article
in En
| MEDLINE
| ID: mdl-27415426
ABSTRACT
Microenvironmental conditions can interfere with the functional role and differentiation of mesenchymal stem cells (MSCs). Recent studies suggest that an inflammatory microenvironment can significantly impact the osteogenic potential of periodontal ligament stem cells (PDLSCs), but the precise effects and mechanisms involved remain unclear. Here, we show for the first time that interleukin-1ß (IL-1ß) has dual roles in the osteogenesis of PDLSCs at concentrations ranging from physiologically healthy levels to those found in chronic periodontitis. Low doses of IL-1ß activate the BMP/Smad signaling pathway to promote the osteogenesis of PDLSCs, but higher doses of IL-1ß inhibit BMP/Smad signaling through the activation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling, inhibiting osteogenesis. These results demonstrate that crosstalk between NF-κB, MAPK and BMP/Smad signaling mediates this dual effect of IL-1ß on PDLSCs. We also show that the impaired osteogenesis of PDLSCs results in more inflammatory cytokines and chemokines being released, inducing the chemotaxis of macrophages, which further clarifies the role of PDLSCs in the pathogenesis of periodontitis.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osteoblasts
/
NF-kappa B
/
P38 Mitogen-Activated Protein Kinases
/
Smad1 Protein
/
Interleukin-1beta
/
Bone Morphogenetic Protein 2
/
Mesenchymal Stem Cells
Limits:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
Language:
En
Journal:
Cell Death Dis
Year:
2016
Document type:
Article
Affiliation country:
China