Effect of fast pH decline during the early postmortem period on calpain activity and cytoskeletal protein degradation of broiler M. pectoralis major.
Poult Sci
; 95(10): 2455-63, 2016 Oct 01.
Article
in En
| MEDLINE
| ID: mdl-27433017
ABSTRACT
The objective of this study was to determine the effects of fast pH decline during the early postmortem period on calpain activity and the degradation of cytoskeletal proteins in broilers. Eighty broilers were randomly categorized into two groups physical restraint (PR) and free struggle (FS). M. pectoralis major (PM) was used for determination of calpain activity, shear value, ultrastructure of myofibrils, and the degradation of desmin, titin, nebulin, and troponin-T. The pH (6.05) of FS group is significantly low than PR group (6.38) at 0.3 h postmortem. Fast pH decline during the early postmortem period led to a decrease of µ/m-calpain activities at 0.3 and 3 h postmortem (P < 0.05), but did not affect the ultimate µ/m-calpain activity. An initial fast decrease in pH increased the degradation of desmin, titin, nebulin, and increased the 30 kDa degradation fragments of troponin-T. Therefore, the fast pH decline during the early postmortem period decreased the µ/m-calpain activity and increased the degradation of cytoskeletal proteins in broiler muscle. It is possible that the fast pH decline experienced an earlier activation of calpains that resulted in earlier protein degradation and ultimately lower shear force.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pectoralis Muscles
/
Calpain
/
Avian Proteins
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Proteolysis
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Meat
Limits:
Animals
Language:
En
Journal:
Poult Sci
Year:
2016
Document type:
Article
Affiliation country:
China