Novel bioactive tetracycline-containing electrospun polymer fibers as a potential antibacterial dental implant coating.
Odontology
; 105(3): 354-363, 2017 Jul.
Article
in En
| MEDLINE
| ID: mdl-27585669
ABSTRACT
The purpose of this investigation was to determine the ability of tetracycline-containing fibers to inhibit biofilm formation of peri-implantitis-associated pathogens [i.e., Porphyromonas gingivalis (Pg), Fusobacterium nucleatum (Fn), Prevotella intermedia (Pi), and Aggregatibacter actinomycetemcomitans (Aa)]. Tetracycline hydrochloride (TCH) was added to a poly(DL-lactide) [PLA], poly(ε-caprolactone) [PCL], and gelatin [GEL] polymer blend solution at distinct concentrations to obtain the following fibers PLAPCL/GEL (TCH-free, control), PLAPCL/GEL + 5 % TCH, PLAPCL/GEL + 10 % TCH, and PLAPCL/GEL + 25 % TCH. The inhibitory effect of TCH-containing fibers on biofilm formation was assessed by colony-forming units (CFU/mL). Qualitative analysis of biofilm inhibition was done via scanning electron microscopy (SEM). Statistical significance was reported at p < 0.05. Complete inhibition of biofilm formation on the fibers was observed in groups containing TCH at 10 and 25 wt%. Fibers containing TCH at 5 wt% demonstrated complete inhibition of Aa biofilm. Even though a marked reduction in CFU/mL was observed with an increase in TCH concentration, Pi proved to be the most resilient microorganism. SEM images revealed the absence of or a notable decrease in bacterial biofilm on the TCH-containing nanofibers. Collectively, our data suggest that tetracycline-containing fibers hold great potential as an antibacterial dental implant coating.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tetracycline
/
Dental Implants
/
Biofilms
/
Coated Materials, Biocompatible
/
Peri-Implantitis
/
Anti-Bacterial Agents
Type of study:
Qualitative_research
Language:
En
Journal:
Odontology
Journal subject:
ODONTOLOGIA
Year:
2017
Document type:
Article
Affiliation country:
Estados Unidos