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Molecular typing of isolates obtained from aborted foetuses in Brucella-free Holstein dairy cattle herd after immunisation with Brucella abortus RB51 vaccine in Egypt.
Wareth, Gamal; Melzer, Falk; Böttcher, Denny; El-Diasty, Mohamed; El-Beskawy, Mohamed; Rasheed, Nesma; Schmoock, Gernot; Roesler, Uwe; Sprague, Lisa D; Neubauer, Heinrich.
Affiliation
  • Wareth G; Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany; Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Egypt. Electronic address: gamal.wareth@fli.de.
  • Melzer F; Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany.
  • Böttcher D; Institute of Pathology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 33, 04103 Leipzig, Germany.
  • El-Diasty M; Animal Health Research Institute-Mansoura Provincial Laboratory, Mansoura, Egypt.
  • El-Beskawy M; Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
  • Rasheed N; Animal Health Research Institute-Mansoura Provincial Laboratory, Mansoura, Egypt.
  • Schmoock G; Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany.
  • Roesler U; Institute of Animal Hygiene and Environmental Health, Free University of Berlin. Robert-von-Ostertag Str. 7-13, 14163 Berlin, Germany.
  • Sprague LD; Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany.
  • Neubauer H; Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany.
Acta Trop ; 164: 267-271, 2016 Dec.
Article in En | MEDLINE | ID: mdl-27664334
ABSTRACT
Bovine brucellosis is endemic in Egypt in spite of application of surveillance and control measures. An increase of abortions was reported in a Holstein dairy cattle herd with 600 animals in Damietta governorate in Egypt after immunisation with Brucella (B.) abortus RB51 vaccine. Twenty one (10.6%) of 197 vaccinated cows aborted after 3 months. All aborted cows had been tested seronegative for brucellosis in the past 3 years. B. abortus was isolated from four foetuses. Conventional biochemical and bacteriological identification and polymerase chain reaction (PCR) confirmed two B. abortus biovar (bv.) 1 smooth and two B. abortus rough strains. None of the B. abortus isolates were identified as RB51. Genotyping analysis by multiple locus of variable number tandem repeats analysis based on 16 markers (MLVA-16) revealed two different profiles with low genetic diversity. B. abortus bv1 was introduced in the herd and caused abortions.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Brucella abortus / Brucellosis, Bovine / Brucella Vaccine / Aborted Fetus / Molecular Typing Type of study: Prognostic_studies Limits: Animals / Pregnancy Country/Region as subject: Africa Language: En Journal: Acta Trop Year: 2016 Document type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Brucella abortus / Brucellosis, Bovine / Brucella Vaccine / Aborted Fetus / Molecular Typing Type of study: Prognostic_studies Limits: Animals / Pregnancy Country/Region as subject: Africa Language: En Journal: Acta Trop Year: 2016 Document type: Article