Purification and Analysis of Male Germ Cells from Adult Mouse Testis.
Methods Mol Biol
; 1510: 159-168, 2017.
Article
in En
| MEDLINE
| ID: mdl-27761820
ABSTRACT
Isolation of pools of spermatogenic cells at specific developmental stages is essential for the investigations of molecular events controlling critical transitions during spermatogenesis. Large-scale cell purification techniques allow for combined proteomics, genomics, and transcriptomics studies. Herein, we describe a procedure for the purification of meiotic and post-meiotic male germ cells from adult mouse testes. We also describe how the fractionated cell populations could be used for further studies. In our laboratory, these protocols are routinely used to specifically investigate the molecular basis of histone acetylation/acylation-driven epigenetic programming.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spermatids
/
Spermatocytes
/
Histones
/
Cell Separation
/
Epigenesis, Genetic
/
Histone Deacetylases
Limits:
Animals
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2017
Document type:
Article
Affiliation country:
Francia