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Cyclooxygenase-2-Derived Prostaglandins Mediate Cerebral Microcirculation in a Juvenile Ischemic Rat Model.
Leger, Pierre-Louis; Pansiot, Julien; Besson, Valerie; Palmier, Bruno; Renolleau, Sylvain; Baud, Olivier; Cauli, Bruno; Charriaut-Marlangue, Christiane.
Affiliation
  • Leger PL; From the PROTECT, INSERM, University Paris Diderot, France (P.-L.L., J.P., V.B., O.B., C.C.-M.); UPMC, AP-HP, Hôpital Armand Trousseau, PICU, Paris, France (P.-L.L.); Pharmacologie de la Circulation Cérébrale-EA4475, University Paris Descartes, Faculté de Pharmacie, France (V.B., B.P.); University R
  • Pansiot J; From the PROTECT, INSERM, University Paris Diderot, France (P.-L.L., J.P., V.B., O.B., C.C.-M.); UPMC, AP-HP, Hôpital Armand Trousseau, PICU, Paris, France (P.-L.L.); Pharmacologie de la Circulation Cérébrale-EA4475, University Paris Descartes, Faculté de Pharmacie, France (V.B., B.P.); University R
  • Besson V; From the PROTECT, INSERM, University Paris Diderot, France (P.-L.L., J.P., V.B., O.B., C.C.-M.); UPMC, AP-HP, Hôpital Armand Trousseau, PICU, Paris, France (P.-L.L.); Pharmacologie de la Circulation Cérébrale-EA4475, University Paris Descartes, Faculté de Pharmacie, France (V.B., B.P.); University R
  • Palmier B; From the PROTECT, INSERM, University Paris Diderot, France (P.-L.L., J.P., V.B., O.B., C.C.-M.); UPMC, AP-HP, Hôpital Armand Trousseau, PICU, Paris, France (P.-L.L.); Pharmacologie de la Circulation Cérébrale-EA4475, University Paris Descartes, Faculté de Pharmacie, France (V.B., B.P.); University R
  • Renolleau S; From the PROTECT, INSERM, University Paris Diderot, France (P.-L.L., J.P., V.B., O.B., C.C.-M.); UPMC, AP-HP, Hôpital Armand Trousseau, PICU, Paris, France (P.-L.L.); Pharmacologie de la Circulation Cérébrale-EA4475, University Paris Descartes, Faculté de Pharmacie, France (V.B., B.P.); University R
  • Baud O; From the PROTECT, INSERM, University Paris Diderot, France (P.-L.L., J.P., V.B., O.B., C.C.-M.); UPMC, AP-HP, Hôpital Armand Trousseau, PICU, Paris, France (P.-L.L.); Pharmacologie de la Circulation Cérébrale-EA4475, University Paris Descartes, Faculté de Pharmacie, France (V.B., B.P.); University R
  • Cauli B; From the PROTECT, INSERM, University Paris Diderot, France (P.-L.L., J.P., V.B., O.B., C.C.-M.); UPMC, AP-HP, Hôpital Armand Trousseau, PICU, Paris, France (P.-L.L.); Pharmacologie de la Circulation Cérébrale-EA4475, University Paris Descartes, Faculté de Pharmacie, France (V.B., B.P.); University R
  • Charriaut-Marlangue C; From the PROTECT, INSERM, University Paris Diderot, France (P.-L.L., J.P., V.B., O.B., C.C.-M.); UPMC, AP-HP, Hôpital Armand Trousseau, PICU, Paris, France (P.-L.L.); Pharmacologie de la Circulation Cérébrale-EA4475, University Paris Descartes, Faculté de Pharmacie, France (V.B., B.P.); University R
Stroke ; 47(12): 3048-3052, 2016 12.
Article in En | MEDLINE | ID: mdl-27834752
ABSTRACT
BACKGROUND AND

PURPOSE:

We previously showed that the selective neuronal nitric oxide synthase inhibitor 7-nitroindazole (7-NI) increases cerebral microcirculation in a juvenile ischemic rat model. We address the roles of cyclooxygenase (COX)-elaborated prostaglandins in collateral recruitment and blood supply.

METHODS:

Fourteen-day-old rats were subjected to ischemia-reperfusion and treated with either PBS or 7-NI (25 mg/kg) at the reperfusion onset. Six-keto-prostaglandin F1α was measured using ELISA. COX-1 and COX-2 and prostaglandin terminal synthesizing enzymes were evaluated using reverse-transcriptase polymerase chain reaction and immunofluorescence. Microvascular blood flow indexes (artery diameter and capillaries number) were measured using sidestream dark-field videomicroscopy in PBS- and 7-NI-treated ischemic rats in the absence or presence of the COX-2 inhibitor NS-398 (5 mg/kg). Cell death was measured with the TUNEL (terminal transferase dUTP nick end labeling) assay and cleaved-caspase-3 immunostaining.

RESULTS:

Six-keto-prostaglandin F1α and COX-2, associated with a prostaglandin E synthase, were significantly increased in PBS- and 7-NI-treated animals 15 minutes and 1 hour after ischemia-reperfusion, respectively. In contrast and as compared with PBS, 7-NI significantly decreased prostacyclin synthase and cytosolic prostaglandins E synthase mRNA. Selective COX-2 inhibition significantly decreased blood flow indexes and significantly reversed the effects of 7-NI, including the number of TUNEL+- and cleaved-caspase-3+-nuclei.

CONCLUSIONS:

These results show that the juvenile rat brains mostly respond to ischemia by a COX-2-dependent prostaglandins production and suggest that the transcriptional responses observed under 7-NI facilitate and reorient COX-2-dependent prostaglandins production.
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Collection: 01-internacional Database: MEDLINE Main subject: 6-Ketoprostaglandin F1 alpha / Reperfusion Injury / Cerebrovascular Circulation / Cyclooxygenase 2 / Cyclooxygenase 2 Inhibitors / Prostaglandin-E Synthases / Microcirculation Limits: Animals Language: En Journal: Stroke Year: 2016 Document type: Article
Search on Google
Add to My VHL
Print
XML
PubMed Links

Collection: 01-internacional Database: MEDLINE Main subject: 6-Ketoprostaglandin F1 alpha / Reperfusion Injury / Cerebrovascular Circulation / Cyclooxygenase 2 / Cyclooxygenase 2 Inhibitors / Prostaglandin-E Synthases / Microcirculation Limits: Animals Language: En Journal: Stroke Year: 2016 Document type: Article