Your browser doesn't support javascript.
loading
Epidemiological Surveillance and Typing Methods to Track Antibiotic Resistant Strains Using High Throughput Sequencing.
Machado, Miguel Paulo; Ribeiro-Gonçalves, Bruno; Silva, Mickael; Ramirez, Mário; Carriço, João André.
Affiliation
  • Machado MP; Instituto de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Alameda Da Universidade, Lisbon, 1649-004, Portugal.
  • Ribeiro-Gonçalves B; Instituto de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Alameda Da Universidade, Lisbon, 1649-004, Portugal.
  • Silva M; Instituto de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Alameda Da Universidade, Lisbon, 1649-004, Portugal.
  • Ramirez M; Instituto de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Alameda Da Universidade, Lisbon, 1649-004, Portugal.
  • Carriço JA; Instituto de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Alameda Da Universidade, Lisbon, 1649-004, Portugal. jcarrico@fm.ul.pt.
Methods Mol Biol ; 1520: 331-356, 2017.
Article in En | MEDLINE | ID: mdl-27873262
ABSTRACT
High-Throughput Sequencing (HTS) technologies transformed the microbial typing and molecular epidemiology field by providing the cost-effective ability for researchers to probe draft genomes, not only for epidemiological markers but also for antibiotic resistance and virulence determinants. In this chapter, we provide protocols for the analysis of HTS data for the determination of multilocus sequence typing (MLST) information and for determining presence or absence of antibiotic resistance genes.
Subject(s)
Key words
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacteria / Drug Resistance, Microbial / Multilocus Sequence Typing / High-Throughput Nucleotide Sequencing / Epidemiological Monitoring Type of study: Screening_studies Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2017 Document type: Article Affiliation country: Portugal
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacteria / Drug Resistance, Microbial / Multilocus Sequence Typing / High-Throughput Nucleotide Sequencing / Epidemiological Monitoring Type of study: Screening_studies Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2017 Document type: Article Affiliation country: Portugal