[Vitamin D metabolism and signaling in human hepatocellular carcinoma and surrounding non-tumorous liver]. / A D-vitamin metabolizmusa humán hepatocellularis carcinomában és az azt körülvevo tumormentes májszövetben.
Orv Hetil
; 157(48): 1910-1918, 2016 Nov.
Article
in Hu
| MEDLINE
| ID: mdl-27889974
ABSTRACT
INTRODUCTION:
1,25-Dihydroxy vitamin D3 mediates antitumor effects in hepatocellular carcinoma.AIM:
We examined mRNA and protein expression differences in 1,25-Dihydroxy vitamin D3-inactivating CYP24A1, mRNA of activating CYP27B1 enzymes, and that of VDR between human hepatocellular carcinoma and surrounding non-tumorous liver.METHODS:
Snap-frozen tissues from 13 patients were studied for mRNA and protein expression of CYP24A1. Paraffin-embedded tissues from 36 patients were used to study mRNA of VDR and CYP27B1. mRNA expression was measured by RT-PCR, CYP24A1 protein was detected by immunohistochemistry.RESULTS:
Expression of VDR and CYP27B1 was significantly lower in hepatocellular carcinoma compared with non-tumorous liver (p<0.05). The majority of the HCC samples expressed CYP24A1 mRNA, but neither of the non-tumorous liver. The gene activation was followed by CYP24A1 protein synthesis.CONCLUSIONS:
The presence of CYP24A1 mRNA and the reduced expression of VDR and CYP27B1 mRNA in human hepatocellular carcinoma samples indicate decreased bioavailability of 1,25-Dihydroxy vitamin D3, providing an escape mechanism from the anti-tumor effect. Orv. Hetil., 2016, 157(48), 1910-1918.Key words
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA, Messenger
/
Gene Expression
/
Carcinoma, Hepatocellular
/
25-Hydroxyvitamin D3 1-alpha-Hydroxylase
/
Liver Neoplasms
Limits:
Humans
Language:
Hu
Journal:
Orv Hetil
Year:
2016
Document type:
Article