On-Chip Magnetic Particle-Based Immunoassays Using Multilaminar Flow for Clinical Diagnostics.
Methods Mol Biol
; 1547: 69-83, 2017.
Article
in En
| MEDLINE
| ID: mdl-28044288
ABSTRACT
Magnetic particles have become popular in recent years for immunoassays due to their high surface-to-volume ratio and the ease of their manipulation. However, such assays also require multiple reaction and washing steps that are both time-consuming and manually laborious. Here, we describe a setup and methodology for performing rapid immunoassays on magnetic particles in continuous flow via their deflection through multiple laminar flow streams of reagents and washing solutions. In particular, we focus on the use of the microfluidic platform for a C-reactive protein (CRP) sandwich immunoassay in less than 60 s.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Immunoassay
/
Microfluidics
/
Microfluidic Analytical Techniques
/
Lab-On-A-Chip Devices
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2017
Document type:
Article
Affiliation country:
Reino Unido