Berberine exerts renoprotective effects by regulating the AGEs-RAGE signaling pathway in mesangial cells during diabetic nephropathy.
Mol Cell Endocrinol
; 443: 89-105, 2017 03 05.
Article
in En
| MEDLINE
| ID: mdl-28087385
ABSTRACT
In this study, we explored the effect of berberine treatment on the AGEs-RAGE pathway in a rat model of diabetic nephropathy, and we investigated the mechanism by which key factors caused kidney injury and the effects of berberine. In vivo, berberine improved fasting blood glucose, body weight, the majority of biochemical and renal function parameters and histopathological changes in the diabetic kidney. Western blotting and immunohistochemistry revealed significant increases in the levels of AGEs, RAGE, P-PKC-ß and TGF-ß1 in injured kidneys, and these levels were markedly decreased by treatment with berberine. In vitro, berberine inhibited mesangial cell proliferation. Cells treated with berberine showed reduced levels of AGEs, accompanied by decreased RAGE, p-PKC and TGF-ß1 levels soon afterwards. Berberine exhibited renoprotective effects in diabetic nephropathy rats, and the molecular mechanism was associated with changes in the levels and regulation of the AGEs-RAGE-PKC-ß-TGF-ß1 signaling pathway.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Berberine
/
Signal Transduction
/
Glycation End Products, Advanced
/
Protective Agents
/
Diabetic Nephropathies
/
Mesangial Cells
/
Receptor for Advanced Glycation End Products
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Mol Cell Endocrinol
Year:
2017
Document type:
Article