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The CCTL (Cpf1-assisted Cutting and Taq DNA ligase-assisted Ligation) method for efficient editing of large DNA constructs in vitro.
Lei, Chao; Li, Shi-Yuan; Liu, Jia-Kun; Zheng, Xuan; Zhao, Guo-Ping; Wang, Jin.
Affiliation
  • Lei C; Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
  • Li SY; Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
  • Liu JK; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Zheng X; Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
  • Zhao GP; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Wang J; Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Nucleic Acids Res ; 45(9): e74, 2017 May 19.
Article in En | MEDLINE | ID: mdl-28115632
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA / Taq Polymerase / CRISPR-Associated Proteins Language: En Journal: Nucleic Acids Res Year: 2017 Document type: Article Affiliation country: China Country of publication: Reino Unido
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA / Taq Polymerase / CRISPR-Associated Proteins Language: En Journal: Nucleic Acids Res Year: 2017 Document type: Article Affiliation country: China Country of publication: Reino Unido