Development of anti-CD47 single-chain variable fragment targeted magnetic nanoparticles for treatment of human bladder cancer.
Nanomedicine (Lond)
; 12(6): 597-613, 2017 Mar.
Article
in En
| MEDLINE
| ID: mdl-28186432
ABSTRACT
AIM:
To develop a novel anti-CD47 single-chain variable fragment (scFv) functionalized magnetic nanoparticles (MNPs) for targeting bladder cell lines and its applicability in thermotherapy. MATERIAL &METHODS:
An immunized murine antibody phage display library was constructed and screened to isolate anti-CD47 binders. A scFv was selected and conjugated to MNPs which was then utilized to discriminate CD47+ bladder cells along with assessing its efficacy in thermotherapy.RESULTS:
An scFv with high affinity to bladder cells was efficiently conjugated to MNPs. Following a hyperthermia treatment, the function of scFv-MNP conjugates led to a considerable reduction in cell viability.CONCLUSION:
The anti-CD47 scFv-MNP conjugate was an effective cancer cell thermotherapy tool that might pave the way for development of bionano-based targeting techniques in both early detection and treatment of cancer.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Urinary Bladder Neoplasms
/
CD47 Antigen
/
Single-Chain Antibodies
/
Magnetite Nanoparticles
Type of study:
Screening_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Nanomedicine (Lond)
Year:
2017
Document type:
Article
Affiliation country:
Irán