The Road to HLA Antibody Evaluation: Do Not Rely on MFI.
Am J Transplant
; 17(6): 1455-1461, 2017 Jun.
Article
in En
| MEDLINE
| ID: mdl-28199773
ABSTRACT
Technological advances in HLA laboratory testing undoubtedly improved the sensitivity and specificity of HLA antibody assessment but not without introducing a set of challenges regarding data interpretation. In particular, the introduction of solid-phase single-antigen bead (SAB) antibody assessment brought the belief that mean fluorescence intensity (MFI) was a quantifiable value. As such, MFI levels heavily influenced HLA antibody reporting, monitoring, and clinical practice. However, given that SAB testing was neither intended for nor approved to be quantifiable, is the use of MFI in current clinical and laboratory practice valid? What, if anything, does this numerical value actually reveal about the pathogenic potential of the antibody? What are the pitfalls and caveats associated with reporting MFI? Herein, we travel the road to HLA antibody assessment and explore the reliability of MFI values to make clinical decisions.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Histocompatibility Testing
/
Kidney Transplantation
/
Fluorescence
/
Graft Rejection
/
HLA Antigens
/
Isoantibodies
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Am J Transplant
Journal subject:
TRANSPLANTE
Year:
2017
Document type:
Article
Affiliation country:
Gabón