Impaired nephrogenesis in neonatal rats with oxygen-induced retinopathy.
Pediatr Int
; 59(6): 704-710, 2017 Jun.
Article
in En
| MEDLINE
| ID: mdl-28207964
ABSTRACT
BACKGROUND:
Preterm neonates are born while nephrogenesis is ongoing, and are commonly exposed to factors in a hyperoxic environment that can impair renal development. Oxidative stress has also been implicated in the development of retinopathy of prematurity (ROP). The rat model of oxygen-induced retinopathy (OIR) is the most clinically relevant model of ROP because its biologic features closely resemble those of ROP in preterm infants. We investigated impaired renal development in a rat model of OIR.METHODS:
Newborn Sprague-Dawley rats were maintained in either a normoxic (room air, 21% O2 ; control group) or a controlled hyperoxic (80% O2 ; OIR group) environment from birth to postnatal day (P) 12. All pups were then raised in room air from P12 to P19.RESULTS:
The hyperoxic environment led to significantly higher urinary excretion of 8-hydroxy-2'-deoxyguanosine, a marker of oxidative DNA damage, and a reduction in nephrogenic zone width at P5 in OIR pups. Additionally, glomerular count was significantly reduced by 20% in the OIR group, and avascular and neovascular changes in the retina were observed only in the OIR group at P19. Messenger RNA levels of vascular endothelial growth factor-A (VEGF-A) and platelet-derived growth factor-ß, essential angiogenic cytokines for glomerulogenesis, in the renal cortex were significantly lower at P5 and significantly higher at P19 in the OIR group compared with controls.CONCLUSION:
Renal impairment was caused by exposure to a hyperoxic environment during nephrogenesis, and the pathology of the impaired nephrogenesis in this OIR model reflects the characteristics of ROP observed in preterm infants.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Retinopathy of Prematurity
/
Hyperoxia
/
Renal Insufficiency
/
Kidney
Type of study:
Etiology_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Animals
Language:
En
Journal:
Pediatr Int
Journal subject:
PEDIATRIA
Year:
2017
Document type:
Article
Affiliation country:
Japón
Publication country:
AU
/
AUSTRALIA
/
AUSTRÁLIA