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Increasing Calcium Level Limits Schwann Cell Numbers In Vitro following Peripheral Nerve Injury.
Yang, Kai J; Yan, Yuhui; Zhang, Lin-Ling; Agresti, Michael A; Matloub, Hani S; LoGiudice, John A; Havlik, Robert; Yan, Ji-Geng.
Affiliation
  • Yang KJ; Department of Plastic Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin.
  • Yan Y; Department of Plastic Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin.
  • Zhang LL; Department of Plastic Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin.
  • Agresti MA; Department of Plastic Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin.
  • Matloub HS; Department of Plastic Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin.
  • LoGiudice JA; Department of Plastic Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin.
  • Havlik R; Department of Plastic Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin.
  • Yan JG; Department of Plastic Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin.
J Reconstr Microsurg ; 33(6): 435-440, 2017 Jul.
Article in En | MEDLINE | ID: mdl-28293917
ABSTRACT
Background After peripheral nerve injury, there is an increase in calcium concentration in the injured nerves. Our previous publications have shown that increase in calcium concentration correlated well with degree of nerve injury and that local infusion of calcitonin has a beneficial effect on nerve recovery. Schwann cells play a pivotal role in regeneration and recovery. We aim to examine cultured Schwann cell survivals in various concentrations of calcium-containing growth media and the effect of calcitonin in such media. Methods To establish baseline in postinjury state, crush injury was induced in male Sprague-Dawley rats' sciatic nerves. Extra- and intraneural calcium concentrations were measured. To study Schwann cell survival, uninjured sciatic nerve segment was harvested and cultured in media containing various amounts of calcium. To study the effect of calcitonin, nerve harvest and culture were done in four additional media (1) normal control, (2) normal control with calcitonin, (3) high calcium medium, and (4) high calcium medium with calcitonin. Schwann cells were studied and analyzed under fluorescent conditions. Results With increasing calcium concentration, there was a significant decrease in the number of Schwann cells. For the experimental groups, in which calcitonin had been added to the growth medium, there were similar amounts of Schwann cells present in both high and low calcium-containing medium. Conclusion Schwann cells are sensitive to increasing calcium concentration. Calcitonin counteracts the detrimental effects of high calcium on Schwann cell survival. This can have significant future clinical implications for patients with peripheral nerve injuries.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Schwann Cells / Sciatic Nerve / Calcium / Nerve Regeneration Type of study: Prognostic_studies Limits: Animals Language: En Journal: J Reconstr Microsurg Journal subject: NEUROCIRURGIA Year: 2017 Document type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Schwann Cells / Sciatic Nerve / Calcium / Nerve Regeneration Type of study: Prognostic_studies Limits: Animals Language: En Journal: J Reconstr Microsurg Journal subject: NEUROCIRURGIA Year: 2017 Document type: Article