Cryopreservation Of Nili-Ravi Buffalo Bull Sperm in Cryodiluent Supplemented with Lolium perenne Protein Preparations.
Cryo Letters
; 38(1): 43-50, 2017.
Article
in En
| MEDLINE
| ID: mdl-28376139
ABSTRACT
BACKGROUND:
Semen from the Nili-Ravi buffalo bull, Bubalus bubalis, shows poor survival after freeze storage compared to bovine (Bos taurus and Bos indicus) semen. Freeze-susceptibility distinctions in these two genera have been attributed to differences in sperm membranes. MATERIALS ANDMETHODS:
We measured the impact of protein preparations derived from a frost-resistant perennial grass, Lolium perenne, with ice recrystallization inhibition activity on the low temperature storage of B. bubalis semen.RESULTS:
When the L. perenne preparations (0.1, 1, 10 µg/mL) were added to buffalo semen [2 ejaculates per bull (N=3) per replicate (r=3)] in Tris-citrate extender (50×106sperm mL-1), there was no impact on semen quality, as measured by sperm motility and plasma membrane integrity, after storage at 4 degree C (P>0.05). However, when semen supplemented with the grass proteins (0.1 and 1 µg mL-1) was evaluated after freezing and storage in liquid nitrogen for 24 h, post-thaw sperm progressive motility and plasma membrane integrity was higher (P<0.05) than in control samples. Post-thaw sperm viability and sperm acrosome integrity was similar (P > 0.05) to controls.CONCLUSION:
The improvement in cryopreserved buffalo sperm progressive motility and plasma membrane integrity suggests that the use of these easily-made preparations may improve fertility after cryopreservation and offers the prospect of improved conception rates after artificial insemination with cryopreservation.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Semen Preservation
/
Lolium
/
Plant Extracts
/
Cryopreservation
/
Cryoprotective Agents
Limits:
Animals
Language:
En
Journal:
Cryo Letters
Journal subject:
BIOLOGIA
/
QUIMICA
Year:
2017
Document type:
Article
Affiliation country:
Pakistán