Targeted drug delivery system to neural cells utilizes the nicotinic acetylcholine receptor.
Int J Pharm
; 525(1): 12-20, 2017 Jun 15.
Article
in En
| MEDLINE
| ID: mdl-28412449
ABSTRACT
Drug delivery to the brain is still a major challenge in the field of therapeutics, especially for large and hydrophilic compounds. In order to achieve drug delivery of therapeutic concentration in the central nervous system, the problematic blood brain barrier (BBB) must be overcome. This work presents the formulation of a targeted nanoparticle-based drug delivery system using a specific neural cell targeting ligand, rabies virus derived peptide (RDP). Characterization studies revealed that RDP could be conjugated to drug-loaded PLGA nanoparticles of average diameter 257.10±22.39nm and zeta potential of -5.51±0.73mV. In vitro studies showed that addition of RDP to nanoparticles enhanced drug accumulation in a neural cell line specifically as opposed to non-neural cell lines. It was revealed that this drug delivery system is reliant upon nicotinic acetylcholine receptor (nAChR) function for RDP-facilitated effects, supporting a cellular uptake mechanism of action. The specific neural cell targeting capabilities of RDP via the nAChR offers a non-toxic, non-invasive and promising approach to the delivery of therapeutics to the brain.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Blood-Brain Barrier
/
Receptors, Nicotinic
/
Drug Delivery Systems
/
Nanoparticles
/
Neurons
Limits:
Humans
Language:
En
Journal:
Int J Pharm
Year:
2017
Document type:
Article
Affiliation country:
Reino Unido