Advances in methods for identification and characterization of plant transporter function.
J Exp Bot
; 68(15): 4045-4056, 2017 07 10.
Article
in En
| MEDLINE
| ID: mdl-28472492
ABSTRACT
Transport proteins are crucial for cellular function at all levels. Numerous importers and exporters facilitate transport of a diverse array of metabolites and ions intra- and intercellularly. Identification of transporter function is essential for understanding biological processes at both the cellular and organismal level. Assignment of a functional role to individual transporter proteins or to identify a transporter with a given substrate specificity has notoriously been challenging. Recently, major advances have been achieved in function-driven screens, phenotype-driven screens, and in silico-based approaches. In this review, we highlight examples that illustrate how new technology and tools have advanced identification and characterization of plant transporter functions.
Key words
Exporter identification; Nitrate and Peptide transporter Family (NPF); Xenopus oocytes; expression cloning; forward genetics transporter identification; in silico approaches for transporter identification; metabolic engineering-based transporter screen; plant transporter identification; receptor- and sensor-based screen; transporter substrate specificity
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Proteins
/
Plants
/
Botany
/
Carrier Proteins
/
Genetic Techniques
Type of study:
Diagnostic_studies
Language:
En
Journal:
J Exp Bot
Journal subject:
BOTANICA
Year:
2017
Document type:
Article
Affiliation country:
Dinamarca