Selection of High-Producing Clones Using FACS for CHO Cell Line Development.
Methods Mol Biol
; 1603: 143-152, 2017.
Article
in En
| MEDLINE
| ID: mdl-28493128
ABSTRACT
Cell line development aims to generate and select clones with desirable characteristics. One of the most important parameters for biopharmaceutical cell selection is cell-specific productivity (Qp) or the quantity of product produced per cell per day. Fluorescence-activated cell sorting (FACS) is a powerful, high-throughput technique that facilitates multiparametric characterization and isolation of individual cell clones from heterogeneous populations. Here, we describe a FACS-based method for section of high-producing CHO cell clones.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Staining and Labeling
/
Cell Separation
/
Flow Cytometry
Limits:
Animals
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2017
Document type:
Article
Affiliation country:
Irlanda