Formulation and characterization of lutetium-177-labeled stannous (tin) colloid for radiosynovectomy.
Nucl Med Commun
; 38(7): 587-592, 2017 Jul.
Article
in En
| MEDLINE
| ID: mdl-28538080
ABSTRACT
OBJECTIVE:
Easy large-scale production, easy availability, cost-effectiveness, long half-life, and favorable radiation characteristics have made lutetium-177 (Lu) a preferred radionuclide for use in therapy. Lutetium-177-labeled stannous (Lu-Sn) colloid particles were formulated for application in radiosynovectomy, followed by in-vitro and in-vivo characterization.METHODS:
Stannous chloride (SnCl2) solution and Lu were heated together, the pH was adjusted, and the particles were recovered by centrifugation. The heating time and amount of SnCl2 were varied to optimize the labeling protocol. The labeling efficiency (LE) and radiochemical purity (RCP) of the product were determined. The size and shape of the particles were determined by means of electron microscopy. In-vitro stability was tested in PBS and synovial fluid, and in-vivo stability was tested in humans.RESULTS:
LE and RCP were greater than 95% and â¼99% (Rf=0-0.1), respectively. Aggregated colloidal particles were spherical (mean size 241±47 nm). The product was stable in vitro for up to 7 days in PBS as well as in synovial fluid. Injection of the product into the infected knee joint of a patient resulted in its homogenous distribution in the intra-articular space, as seen on the scan. No leakage of activity was seen outside the knee joint even 7 days after injection, indicating good tracer binding and in-vivo stability.CONCLUSION:
Lu-Sn colloid was successfully prepared with a high LE (>95%) and high RCP (99%) under optimized reaction conditions. Because of the numerous benefits of Lu and the ease of preparation of tin colloid particles, Lu-Sn colloid particles are significantly superior to its currently available counterparts for use in radiosynovectomy.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Radioisotopes
/
Radiosurgery
/
Tin Compounds
/
Lutetium
Type of study:
Guideline
Limits:
Humans
Language:
En
Journal:
Nucl Med Commun
Year:
2017
Document type:
Article
Affiliation country:
India