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The Methods of Choice for Extracellular Vesicles (EVs) Characterization.
Szatanek, Rafal; Baj-Krzyworzeka, Monika; Zimoch, Jakub; Lekka, Malgorzata; Siedlar, Maciej; Baran, Jarek.
Affiliation
  • Szatanek R; Department of Clinical Immunology, Institute of Paediatrics, Jagiellonian University Medical College, 30-663 Krakow, Poland. rafal.szatanek@uj.edu.pl.
  • Baj-Krzyworzeka M; Department of Clinical Immunology, Institute of Paediatrics, Jagiellonian University Medical College, 30-663 Krakow, Poland. mibaj@cyf-kr.edu.pl.
  • Zimoch J; Tissue Biology Research Unit, Department of Surgery, University Children's Hospital Zurich, 8032 Zurich, Switzerland. jakub.zimoch@kispi.uzh.ch.
  • Lekka M; Department of Research of Biophysical Microstructure, Institute of Nuclear Physics Polish Academy of Sciences, 31-342 Krakow, Poland. malgorzata.lekka@gmail.com.
  • Siedlar M; Department of Clinical Immunology, Institute of Paediatrics, Jagiellonian University Medical College, 30-663 Krakow, Poland. misiedla@cyf-kr.edu.pl.
  • Baran J; Department of Clinical Immunology, Institute of Paediatrics, Jagiellonian University Medical College, 30-663 Krakow, Poland. mibaran@cyf-kr.edu.pl.
Int J Mol Sci ; 18(6)2017 May 29.
Article in En | MEDLINE | ID: mdl-28555055
In recent years, extracellular vesicles (EVs) have become a subject of intense study. These membrane-enclosed spherical structures are secreted by almost every cell type and are engaged in the transport of cellular content (cargo) from parental to target cells. The impact of EVs transfer has been observed in many vital cellular processes including cell-to-cell communication and immune response modulation; thus, a fast and precise characterization of EVs may be relevant for both scientific and diagnostic purposes. In this review, the most popular analytical techniques used in EVs studies are presented with the emphasis on exosomes and microvesicles characterization.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Extracellular Vesicles Limits: Animals / Humans Language: En Journal: Int J Mol Sci Year: 2017 Document type: Article Affiliation country: Polonia Country of publication: Suiza
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Extracellular Vesicles Limits: Animals / Humans Language: En Journal: Int J Mol Sci Year: 2017 Document type: Article Affiliation country: Polonia Country of publication: Suiza