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Regional distribution of rat brain alpha 1-adrenergic receptors: correlation between (125I)-heat membrane binding and in vitro autoradiography.
Life Sci ; 36(1): 45-51, 1985 Jan 07.
Article in En | MEDLINE | ID: mdl-2857045
[125I]-HEAT has proven useful for in vitro autoradiography as a specific alpha 1-adrenergic radioligand. We compared the binding of [125I]-HEAT to membranes from ten brain regions with the densitometric readings of these regions in autoradiographs. There was an excellent correlation between receptor numbers from membrane binding and relative optical densities from the autoradiography. The affinity of HEAT for binding to membranes from various regions was similar. The results of this direct comparison are further evidence that HEAT binds to alpha 1-adrenergic receptors in lightly fixed tissue sections. A further interesting observation is that in regions with a heterogeneous distribution of binding sites, membrane binding may not reflect the presence of a dense local population of receptors.
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Collection: 01-internacional Database: MEDLINE Main subject: Brain Chemistry / Receptors, Adrenergic, alpha / Tetralones Limits: Animals Language: En Journal: Life Sci Year: 1985 Document type: Article Country of publication: Países Bajos
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Collection: 01-internacional Database: MEDLINE Main subject: Brain Chemistry / Receptors, Adrenergic, alpha / Tetralones Limits: Animals Language: En Journal: Life Sci Year: 1985 Document type: Article Country of publication: Países Bajos