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Establishment of a rabbit induced pluripotent stem cell (RbiPSC) line using lentiviral delivery of human pluripotency factors.
Táncos, Zsuzsanna; Nemes, Csilla; Varga, Eszter; Bock, István; Rungarunlert, Sasitorn; Tharasanit, Theerawat; Techakumphu, Mongkol; Kobolák, Julianna; Dinnyés, András.
Affiliation
  • Táncos Z; Molecular Animal Biotechnology Laboratory, Szent István University, Gödöllo, Hungary; BioTalentum Ltd., Gödöllo, Hungary.
  • Nemes C; BioTalentum Ltd., Gödöllo, Hungary.
  • Varga E; Molecular Animal Biotechnology Laboratory, Szent István University, Gödöllo, Hungary; BioTalentum Ltd., Gödöllo, Hungary.
  • Bock I; Molecular Animal Biotechnology Laboratory, Szent István University, Gödöllo, Hungary; BioTalentum Ltd., Gödöllo, Hungary.
  • Rungarunlert S; Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, Thailand.
  • Tharasanit T; Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
  • Techakumphu M; Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
  • Kobolák J; BioTalentum Ltd., Gödöllo, Hungary.
  • Dinnyés A; Molecular Animal Biotechnology Laboratory, Szent István University, Gödöllo, Hungary; BioTalentum Ltd., Gödöllo, Hungary; Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. Electronic address: andras.dinnyes@biotalentum.hu.
Stem Cell Res ; 21: 16-18, 2017 05.
Article in En | MEDLINE | ID: mdl-28677530
Rabbit Embryonic Fibroblast (RbEF) cells (from Hycole hybrid rabbit foetus) were reprogrammed by lentiviral delivery of a self-silencing hOKSM polycistronic vector. The pluripotency of the newly generated RbiPSC was verified by the expression of pluripotency-associated markers and by in vitro spontaneous differentiation towards the 3 germ layers. Furthermore, the spontaneous differentiation potential of the iPSC was also tested in vivo by teratoma assay. The iPSC line showed normal karyotype. The advantages of using RbiPSC are the easy access to primary material and the possibility to study the efficacy and safety of the iPSC-based therapies on a non-rodent animal model.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Transcription Factors / Transduction, Genetic / Lentivirus / Induced Pluripotent Stem Cells Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Stem Cell Res Year: 2017 Document type: Article Affiliation country: Hungria Country of publication: Reino Unido
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Transcription Factors / Transduction, Genetic / Lentivirus / Induced Pluripotent Stem Cells Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Stem Cell Res Year: 2017 Document type: Article Affiliation country: Hungria Country of publication: Reino Unido