Overexpression of a novel MYB-related transcription factor, OsMYBR1, confers improved drought tolerance and decreased ABA sensitivity in rice.
Biochem Biophys Res Commun
; 490(4): 1355-1361, 2017 09 02.
Article
in En
| MEDLINE
| ID: mdl-28690154
ABSTRACT
The MYB proteins play important roles in regulating plant responses to environmental stresses. We cloned and functionally characterized a novel MYB-related gene, OsMYBR1, from rice. Our microarray and qRT-PCR analyses showed that its expression was induced by drought and cold in different tissues at various developmental stages. This gene encodes a putative MYB-related protein of 463 amino acid residues. Compared with wild-type (WT) plants, transgenic plants over-expressing OsMYBR1 exhibited much greater tolerance to drought stress and decreased sensitivity to abscisic acid (ABA). Under drought treatment, levels of free proline and soluble sugar were higher in transgenic plants than in the WT. Furthermore, transcriptional expression of four stress-related genes -- OsP5CS1, OsProt, OsLEA3, and OsRab16 -- was significantly increased in transgenic plants under drought stressed conditions and ABA. Our results provide evidence that OsMYBR1 is involved in mediating plant responses to ABA and drought.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oryza
/
Transcription Factors
/
Adaptation, Physiological
/
Plants, Genetically Modified
/
Abscisic Acid
/
Gene Expression Regulation, Plant
Type of study:
Diagnostic_studies
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2017
Document type:
Article