Towards sensitive, high-throughput, biomolecular assays based on fluorescence lifetime.
Methods Appl Fluoresc
; 5(3): 034002, 2017 09 22.
Article
in En
| MEDLINE
| ID: mdl-28699919
ABSTRACT
Time-resolved fluorescence detection for robust sensing of biomolecular interactions is developed by implementing time-correlated single photon counting in high-throughput conditions. Droplet microfluidics is used as a promising platform for the very fast handling of low-volume samples. We illustrate the potential of this very sensitive and cost-effective technology in the context of an enzymatic activity assay based on fluorescently-labeled biomolecules. Fluorescence lifetime detection by time-correlated single photon counting is shown to enable reliable discrimination between positive and negative control samples at a throughput as high as several hundred samples per second.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biological Assay
/
Microfluidic Analytical Techniques
/
Fluorescence
Type of study:
Diagnostic_studies
Language:
En
Journal:
Methods Appl Fluoresc
Year:
2017
Document type:
Article
Affiliation country:
Francia