Transformation-induced changes in the DNA-nuclear matrix interface, revealed by high-throughput analysis of DNA halos.
Sci Rep
; 7(1): 6475, 2017 07 25.
Article
in En
| MEDLINE
| ID: mdl-28743923
ABSTRACT
In higher eukaryotic nuclei, DNA is periodically anchored to an extraction-resistant protein structure, via matrix attachment regions. We describe a refined and accessible method to non-subjectively, rapidly and reproducibly measure both size and stability of the intervening chromatin loops, and use it to demonstrate that malignant transformation compromises the DNA-nuclear matrix interface.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA
/
Nuclear Matrix
/
High-Throughput Screening Assays
Limits:
Humans
Language:
En
Journal:
Sci Rep
Year:
2017
Document type:
Article
Affiliation country:
Reino Unido
Publication country:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM